Kubva muna Ndira 2020 kusvika Gumiguru 2020, ongororo yemaitiro yakaitwa paPisa University Hospital iyo yakaburitswa paBMC Microbiology.Goldstream®Fungus (1-3) -β-D-Glucan Test yakashandiswa pakuona chiyero cheBDG kubva kuBAL samples.Mhedzisiro yacho yakaverengerwa naYakazara Otomatiki Kinetic Tube Reader IGL-200kubva kuEra Biology.Tsvagiridzo inoratidza kuti BDG inofanirwa kucherechedzwa nekuda kwayo yakakwira yakaipa yekufungidzira kukosha.Uye zvakaguma zvakakosha mukutonga kunze kwekuongororwa kwePCP kune vese vasina kunaka vanodzora varwere.
Background:
Ikozvino yekuongorora goridhe mwero wePneumocystis jiroveciiinomiririrwa ne microscopic visualization yefungus kubva kukiriniki yekufema samples, sebronchoalveolar-lavage fluid, inotsanangura "kuratidzwa"P. jiroveciipneumonia, nepo qPCR inobvumira kutsanangura "zvinogoneka" kuongororwa, sezvo isingakwanise kusarura hutachiona kubva mukoloni.Zvisineyi, nzira dzemamorekuru, dzakaita semagumo-poinzi PCR neqPCR, dzinokurumidza, dziri nyore kuita uye kududzira, nokudaro zvichibvumira marabhoritari kudzosa kuna chiremba ruzivo runobatsira rwemicrobiological munguva pfupi.Chidzidzo chazvino chine chinangwa chekuenzanisa microscopy nema molecular assays uye beta-D-glucan diagnostic performance pane bronchoalveolar-lavage fluid kubva kuvarwere vanofungidzirwa.Pneumocystis jiroveciichirwere chemabayo.Bronchoalveolar-lavage fluid kubva gumi nesere-yakanyanya-ngozi uye ina isina kunaka yekudzora zvidzidzo zvakasangana neGrocott-Gomori's methenamine silver-staining, end-point PCR, RT-PCR, uye beta-D-glucan assay.
Mhinduro:
Yese microscopically positive bronchoalveolar-lavage samples (50%) yakakonzerawo yakanaka nekuguma-point uye chaiyo nguva PCR uye zvese, asi zviviri, zvakakonzera zvakanaka zvakare nebeta-D-glucan quantification.End-point PCR uye RT-PCR yakaona 10 (55%) uye 11 (61%) kubva mumasampuli gumi nemasere, zvichiteerana, zvichiratidza kunzwisiswa kwakawedzera mukuenzanisa nemicroscope.Yese RT-PCR ine Ct <27 yakasimbiswa microscopically, nepo samples ine Ct≥ 27 yakanga isiri.
Mhedziso:
Basa redu rinoratidza kukosha kwekugadzirisa zvakare uye kutsanangura patsva basa rekuongorora mamorekuru mune yakasarudzika kiriniki mamiriro, senge.P. jiroveciihutachiona, hunove husingawanzo asi zvakare hwakakomba uye nekukurumidza kufambira mberi kwekiriniki mamiriro anokanganisa immunocompromised mauto ayo anogona kunyanya kubatsirwa kubva nekukurumidza kuongororwa.Vakanyatsosarudzwa varwere, maererano nemaitiro ekubatanidza, zvinoguma zvisina kunaka nenzira dzema molecular zvinogona kutongerwa kunzeP. jiroveciichirwere chemabayo.
Franconi I, Leonildi A, Erra G, et al.Kuenzanisa kwakasiyana microbiological maitiro ekuongororwa kwePneumocystis jirovecii pneumonia pane bronchoalveolar-lavage fluid.BMC Microbiol.2022;22(1):143.Yakabudiswa 2022 Chivabvu 21. doi:10.1186/s12866-022-02559-1
Nguva yekutumira: Jun-14-2022