Aya akatevedzana enzira kuyedza nekushandisa chaiyo hutachiona antigen kuona masoja ekudzivirira chirwere muserum yevarwere, kusanganisira kuona masoja ekudzivirira chirwere eIgM uye kuyerwa kwemasoja ekudzivirira chirwere eIgG.Masoja ekudzivirira chirwere eIgM anonyangarika mumavhiki akati wandei, nepo masoja ekudzivirira chirwere eIgG achienderera mberi kwemakore mazhinji.Kumisikidza kuongororwa kwehutachiona hwehutachiona kunoitwa serologically nekuratidza kukwira kweiyo antibody titer kuhutachiona kana nekuratidzira antiviral antibodies ekirasi yeIgM.Nzira dzakashandiswa dzinosanganisira bvunzo dzeNeutralization (Nt), the complement fixation (CF) test, hemagglutination inhibition (HI) test, uye immunofluorescence (IF) test, passive hemagglutination, uye immunodiffusion.
A. Neutralization Assays
Munguva yehutachiona kana sero tsika, hutachiona hunogona kuvhariswa neayo chaiwo antibody uye kurasikirwa nekutapukira, rudzi urwu rweantibody runotsanangurwa se neutralization antibody.Neutralization assays ndeyekuona iyo neutralization antibody muserum yevarwere.
B. Complement Fixation Assays
Iyo yekuwedzera gadziriso assay inogona kushandiswa kutarisa kuvepo kweiyo chaiyo antibody kana antigen muserum yemurwere.Muedzo unoshandisa masero matsvuku eropa (SRBC), anti-SRBC antibody uye anokwana, pamwe chete neantigen chaiyo (kana ichitsvaga antibody muserum) kana chaiyo antibody (kana ichitsvaga antigen muserum).
C. Hemagglutination Inhibition Assays
Kana huwandu hwehutachiona mumuenzaniso hwakakwira, kana sampu yakasanganiswa neRBCs, lattice yehutachiona uye RBCs ichaumbwa.Ichi chiitiko chinonzi hemagglutination.Kana masoja ekudzivirira chirwere anorwisa hemagglutinins aripo, hemagglutination inodzivirirwa.Munguva yehemagglutination inhibition test, serial dilutions ye serum inosanganiswa nehuwandu hunozivikanwa hwehutachiona.Mushure mekuita, maRBC anowedzerwa, uye musanganiswa unosiiwa kuti ugare kwemaawa akawanda.Kana hemagglutination ikadziviswa, pellet yeRBCs inoumbwa pazasi pechubhu.Kana hemagglutination isingadzivirirwe, firimu yakaonda inoumbwa.
Nguva yekutumira: Oct-15-2020